European Union, European Centre for Disease Prevention and Control. 04/27/2018 This four-page report is based on data for 2015 retrieved from the European Surveillance System (TESSy). In 2015, nine anthrax cases were reported by three European Union/European Economic Area countries. Four of these cases were confirmed; the remaining 27 reporting countries notified zero cases. The report discusses methods, epidemiology, threats description for 2015, and public health implications. (PDF)

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We describe the reshaping of siderocalin by combinatorial and rational protein engineering to specifically bind PB*FeIII instead of BB*FeIII, and with even higher affinity (KD ≈ 20 pM). X-ray crystallographic analysis of the resulting “petrocalin” in complex with PB*GaIII reveals a positively charged ligand pocket dominated by two Lys and two Arg side chains, of which Arg100 also forms two hydrogen bonds to the central carboxyl and hydroxyl groups of PB. Furthermore, the extended butterfly-like conformation of the PB metal-chelate complex, first elucidated in this study, provides a rationale for the missing…

Source: Angewandte ChemieCategory: Chemistry Authors: Tags: Angew Chem Int Ed Engl Source Type: research

This study may contribute to understanding the mechanisms under which sequences evolve in their structures and how evolutionary processes enable structural variations.

Source: GenesCategory: Genetics & Stem Cells Authors: Tags: Article Source Type: research

Volume 9, Issue 1, 2018, Page 1085-1091 .

Source: VirulenceCategory: Virology Authors: Source Type: research

Abstract
Detecting bacterial cells at low levels is critical in public health, the food industry and first response. Current processes typically involve laborious cell lysis and genomic DNA extraction to achieve 100-1000 CFU mL-1 levels for detecting gram-positive bacteria. As an alternative to DNA-based methods, cell wall binding domains (CBDs) derived from lysins having a modular structure with an N-terminal catalytic domain and a C-terminal CBD, can be used to detect bacterial pathogens as a result of their exceptionally specific binding to target bacteria with great avidity. We have developed a highly sens…

Source: Analytica Chimica ActaCategory: Chemistry Authors: Tags: Anal Chim Acta Source Type: research

Publication date: Available online 30 May 2018Source: Biochimica et Biophysica Acta (BBA) – General SubjectsAuthor(s): Basavraj Khanppnavar, Saumen DattaAbstractBackgroundThe nucleotidyl cyclase toxin ExoY is an important virulence determinant of Pseudomonas aeruginosa that causes severe acute and chronic infections in immune-compromised individuals. Additionally, this unique T3SS effector shows a striking preference for cUMP, a newly identified non-canonical secondary messenger. Thereby, ExoY is also considered as a potential tool to study unexplored cUMP signaling pathways.MethodsThe crystal structure of ExoY was determi…

Publication date: 2018Source: MethodsX, Volume 5Author(s): Meera J. Patel, Gulden Yilmaz, Lavesh Bhatia, Esther E. Biswas-Fiss, Subhasis B. BiswasAbstractFluorescence Resonance Energy Transfer (FRET) is a well-known methodology for detection and quantitation of structural changes of proteins in solution. FRET requires site-specific protein labeling with two fluorophores, one of which functions as an energy donor and the other one as an energy acceptor. However, the site-specific labeling of protein is often complex and difficult, particularly when inserting two fluorophores in specific sites. We have examined several prote…

Source: MethodsXCategory: Science Source Type: research

Publication date: 5 September 2018Source: Journal of Hazardous Materials, Volume 357Author(s): Hua Fang, Lingxi Han, Houpu Zhang, Zhengnan Long, Lin Cai, Yunlong YuAbstractThe dissemination of antibiotic resistance genes (ARGs), human pathogenic bacteria (HPB), and antibiotic-resistant HPB (ARHPB) from animal feedlot to nearby environment poses a potentially high risk to environmental ecology and public health. Here, a metagenomic analysis was employed to explore the dissemination of ARGs, HPB, and ARHPB from a pig feedlot to surrounding stream and agricultural soils. In total, not detectable (ND)-1,628.4 μg/kg of ant…

Publication date: June 2018Source: Meta Gene, Volume 16Author(s): P.N. Shivakumara, T.V. Aravindakshan, Thomas Naicy, K. Anilkumar, R. UmaAbstractToll like receptors (TLRs) are type 1 transmembrane proteins expressed in almost all cell types and activate the innate immune system. The present study involved characterization and differential transcriptional expression profiling of Toll-like receptor-2 gene in Vechur (Bos indicus) and crossbred (Bos indicus X Bos taurus) cattle of Kerala in response to anthrax vaccination. Sequence analysis revealed 2355 bp long TLR2 open reading frame encoding 784 amino acids with a signal…

Source: Meta GeneCategory: Genetics & Stem Cells Source Type: research

Publication date: August 2018Source: Journal of Microbiological Methods, Volume 151Author(s): Yan-chun Wang, Li-si Yuan, Hao-xia Tao, Wei Jiang, Chun-jie LiuAbstractSeveral genetic tools have been developed for use in Bacillus anthracis, but there is still a need for a more marker-free gene inactivation protocols. Thus, we report a method to generate unmarked mutations in B. anthracis. This approach was based on the counter-selectable pheS* gene with assistance by the I-SceI homing endonuclease. Using this strategy, the NprR gene, a transcriptional activator of B. anthracis, was deleted at an extremely high efficiency. Our…

Bacillus endophyticus is a soil plant-endophytic bacterium, while B. anthracis is the causative agent of anthrax. The virulence factors of B. anthracis are the plasmid encoded tripartite toxins (pXO1) and poly- γ-…

Source: BMC MicrobiologyCategory: Microbiology Authors: Tags: Research article Source Type: research





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