Important for Gene Expression Studies.I have posted many examples of how our customers usei-FectTM  and otherTransfection Solutions for Gene Manipulation Studies. There are also manypublications.Here we feature how i-Fect was used to delivery plasmids to the CNS: Sara Elramah, Mar ía José López-González, Matthieu Bastide, Florence Dixmérias, Olivier Roca-Lapirot, Anne-Cécile Wielanek-Bachelet, Anne Vital, Thierry Leste-Lasserre, Alexandre Brochard, Marc Landry&Alexandre Favereaux.Spinal miRNA-124 regulates synaptopodin and nociception in an animal model of bone cancer pain. Scientific Reports 7, Article number: 10949 (2017) doi:10.1038/s41598-017-10224-1…Intrathecal administration of miRNAs and ShRNA To over-express miR-124, we cloned the pre-miRNA sequence of miR-124 into a plasmid. To determine cells expressing this miR-124 encoding plasmid, we added a GFP-coding sequence to the construct under the control of an IRES. Thus, miR-124 over-expressing cells also express GFP. To inhibit synaptopodin expression, we cloned a ShRNA sequence directed against synaptopodin into a plasmid. To determine cells expressing this ShRNA, we added a GFP-coding sequence to the construct under the control of an IRES. Thus, ShRNA expressing cells also expressed GFP. Two micrograms of these plasmids or the corresponding controls, were solubilized in 10 µl of i-Fect reagent (Neuromics, Edina, USA), and injected intrathecally betwe…

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Publication date: Available online 6 July 2018Source: Pharmacological ResearchAuthor(s): Ya-Qun Zhou, Dai-Qiang Liu, Shu-Ping Chen, Jia Sun, Xiao-Mei Wang, Yu-Ke Tian, Wei Wu, Da-Wei YeAbstractChronic pain remains to be a clinical challenge due to insufficient therapeutic strategies. Minocycline is a member of the tetracycline class of antibiotics, which has been used in clinic for decades. It is frequently reported that minocycline may has many non-antibiotic properties, among which is its anti-nociceptive effect. The results from our lab and others suggest that minocycline exerts strong analgesic effect in in animal mode…

ConclusionPediatric neck pain and/or torticollis should raise high suspicion for malignancy of cervical spine. Modern diagnostic means and techniques can assist in the screening and diagnosis of these tumors.

Publication date: Available online 6 July 2018Source: Pharmacological ResearchAuthor(s): Ya-Qun Zhou, Dai-Qiang Liu, Shu-Ping Chen, Jia Sun, Xiao-Mei Wang, Yu-Ke Tian, Wei Wu, Da-Wei YeAbstractChronic pain remains to be a clinical challenge due to insufficient therapeutic strategies. Minocycline is a member of the tetracycline class of antibiotics, which has been used in clinic for decades. It is frequently reported that minocycline may has many non-antibiotic properties, among which is its anti-nociceptive effect. The results from our lab and others suggest that minocycline exerts strong analgesic effect in in animal mode…

ConclusionPediatric neck pain and/or torticollis should raise high suspicion for malignancy of cervical spine. Modern diagnostic means and techniques can assist in the screening and diagnosis of these tumors.

This study evaluated its antihypersensitivity activity in the rat models of neuropathic and cancer pains and explored its underlying mechanisms. Subcutaneous injection of cumulative doses of lappaconitine produced dose-dependent mechanical antiallodynia and thermal antihyperalgesia in spina l nerve ligation-induced neuropathic rats. The cumulative dose–response analysis exhibited theirEmax values of 53.3 and 58.3% MPE, and ED50 values of 1.1 and 1.6  mg/kg. Single intrathecal lappaconitine dose in neuropathy also dose- and time-dependently blocked mechanical allodynia, with anEmax of 66.1% MPE and an ED50 of 0.8…

Source: Journal of Pain ResearchCategory: Anesthesiology Tags: Journal of Pain Research Source Type: research

Agonists of the vanilloid receptor transient vanilloid potential 1 (TRPV1) are emerging as highly efficacious nonopioid analgesics in preclinical studies. These drugs selectively lesion TRPV1+ primary sensory afferents, which are responsible for the transmission of many noxious stimulus modalities. Resiniferatoxin (RTX) is a very potent and selective TRPV1 agonist and is a promising candidate for treating many types of pain. Recent work establishing intrathecal application of RTX for the treatment of pain resulting from advanced cancer has demonstrated profound analgesia in client-owned dogs with osteosarcoma. The present …

Source: Journal of Clinical InvestigationCategory: Biomedical Science Authors: Source Type: research

Conclusion Pediatric neck pain and/or torticollis should raise high suspicion for malignancy of cervical spine. Modern diagnostic means and techniques can assist in the screening and diagnosis of these tumors.

CONCLUSION: Pediatric neck pain and/or torticollis should raise high suspicion for malignancy of cervical spine. Modern diagnostic means and techniques can assist in the screening and diagnosis of these tumors.
PMID: 29544873 [PubMed – as supplied by publisher]

Source: Neurologia i Neurochirurgia PolskaCategory: Neurology Authors: Tags: Neurol Neurochir Pol Source Type: research

Publication date: Available online 23 February 2018 Source:Neuroscience Author(s): Xinran Hou, Yingqi Weng, Tongxuan Wang, Bihan Ouyang, Yalin Li, Zongbin Song, Yundan Pan, Zhong Zhang, Wangyuan Zou, Changsheng Huang, Qulian Guo Epigenetic modulation participates in the mechanism of multiple types of pathological pain, so targeting the involved regulators may be a promising strategy for pain treatment. Our previous research identified the analgesic effect of the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) on mechanical hyperalgesia in a rat model of bone cancer pain (BCP) via restoration of μ-opioid recep…

Source: NeuroscienceCategory: Neuroscience Source Type: research





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