U.S. Environmental Protection Agency, Office of Research and Development, National Homeland Security Research Center. 02/2017 This 28-page protocol describes the processing steps for 45g and 9g soil samples potentially contaminated with Bacillus anthracis spores. The protocol is designed to separate and concentrate the spores from bulk soil down to a pellet that can be used for further analysis. Soil extraction solution and mechanical shaking are used to disrupt soil particle aggregates and to aid in the separation of spores from soil particles. (PDF)

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Condition:   Therapeutic Treatment of Inhalation Anthrax Intervention:   Biological: Raxibacumab Sponsors:   Human Genome Sciences Inc., a GSK Company;   GlaxoSmithKline Completed

Source: ClinicalTrials.govCategory: Research Source Type: clinical trials

Publication date: Available online 9 August 2018Source: Sensors and Actuators B: ChemicalAuthor(s): Abdellatif Ait Lahcen, Fabiana Arduini, Florigio Lista, Aziz AmineAbstractHerein, we report a simple, cost-effective, selective, and rapid electrochemical sensor for Bacillus cereus spore detection, used as simulant for Bacillus anthracis spores. The sensor was developed using carbon paste electrode functionalized with imprinted polymer, fabricated by pyrrole electropolymerization in presence of a known amount of spores as template onto the surface of working electrochemical sensor. The measurement encompasses the cyclic vol…

A pig at a smallholding in Romania has contracted anthrax, which kills thousands of livestock in tropical and sub-tropical areas each year but is rare in temperate regions.

Source: Reuters: HealthCategory: Consumer Health News Tags: healthNews Source Type: news

We describe the reshaping of siderocalin by combinatorial and rational protein engineering to specifically bind PB*FeIII instead of BB*FeIII, and with even higher affinity (KD ≈ 20 pM). X-ray crystallographic analysis of the resulting “petrocalin” in complex with PB*GaIII reveals a positively charged ligand pocket dominated by two Lys and two Arg side chains, of which Arg100 also forms two hydrogen bonds to the central carboxyl and hydroxyl groups of PB. Furthermore, the extended butterfly-like conformation of the PB metal-chelate complex, first elucidated in this study, provides a rationale for the missing…

Source: Angewandte ChemieCategory: Chemistry Authors: Tags: Angew Chem Int Ed Engl Source Type: research

This study may contribute to understanding the mechanisms under which sequences evolve in their structures and how evolutionary processes enable structural variations.

Source: GenesCategory: Genetics & Stem Cells Authors: Tags: Article Source Type: research

Volume 9, Issue 1, 2018, Page 1085-1091 .

Source: VirulenceCategory: Virology Authors: Source Type: research

Detecting bacterial cells at low levels is critical in public health, the food industry and first response. Current processes typically involve laborious cell lysis and genomic DNA extraction to achieve 100-1000 CFU mL-1 levels for detecting gram-positive bacteria. As an alternative to DNA-based methods, cell wall binding domains (CBDs) derived from lysins having a modular structure with an N-terminal catalytic domain and a C-terminal CBD, can be used to detect bacterial pathogens as a result of their exceptionally specific binding to target bacteria with great avidity. We have developed a highly sens…

Source: Analytica Chimica ActaCategory: Chemistry Authors: Tags: Anal Chim Acta Source Type: research

Publication date: Available online 30 May 2018Source: Biochimica et Biophysica Acta (BBA) – General SubjectsAuthor(s): Basavraj Khanppnavar, Saumen DattaAbstractBackgroundThe nucleotidyl cyclase toxin ExoY is an important virulence determinant of Pseudomonas aeruginosa that causes severe acute and chronic infections in immune-compromised individuals. Additionally, this unique T3SS effector shows a striking preference for cUMP, a newly identified non-canonical secondary messenger. Thereby, ExoY is also considered as a potential tool to study unexplored cUMP signaling pathways.MethodsThe crystal structure of ExoY was determi…

Publication date: 2018Source: MethodsX, Volume 5Author(s): Meera J. Patel, Gulden Yilmaz, Lavesh Bhatia, Esther E. Biswas-Fiss, Subhasis B. BiswasAbstractFluorescence Resonance Energy Transfer (FRET) is a well-known methodology for detection and quantitation of structural changes of proteins in solution. FRET requires site-specific protein labeling with two fluorophores, one of which functions as an energy donor and the other one as an energy acceptor. However, the site-specific labeling of protein is often complex and difficult, particularly when inserting two fluorophores in specific sites. We have examined several prote…

Source: MethodsXCategory: Science Source Type: research

Publication date: 5 September 2018Source: Journal of Hazardous Materials, Volume 357Author(s): Hua Fang, Lingxi Han, Houpu Zhang, Zhengnan Long, Lin Cai, Yunlong YuAbstractThe dissemination of antibiotic resistance genes (ARGs), human pathogenic bacteria (HPB), and antibiotic-resistant HPB (ARHPB) from animal feedlot to nearby environment poses a potentially high risk to environmental ecology and public health. Here, a metagenomic analysis was employed to explore the dissemination of ARGs, HPB, and ARHPB from a pig feedlot to surrounding stream and agricultural soils. In total, not detectable (ND)-1,628.4 μg/kg of ant…

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